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oligod t 25 magnetic beads  (New England Biolabs)


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  • 98

    Structured Review

    New England Biolabs oligod t 25 magnetic beads
    Oligod T 25 Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 985 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/oligod+t+25+magnetic+beads/Oligo+d(T)25+Magnetic+Beads/pm41222267-92-11-14
    Average 98 stars, based on 985 article reviews
    oligod t 25 magnetic beads - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Selection:

    Article Title: Sex-specific transcriptional regulation in lung macrophages during sub-acute Mycobacterium tuberculosis infection.
    Article Snippet: The quality of RNA was first checked for integrity on an Agilent TapeStation; samples with RNA integrity number (RIN) >7.0 were used for subsequent processing. .. Total RNA was subjected to two rounds of poly(A) selection using oligod(T)25 magnetic beads (New England Biolabs). .. An Illumina-compatible RNA-seq library was prepared using NEBNext Ultra RNA-seq Library Preparation Kit.

    Magnetic Beads:

    Article Title: Sex-specific transcriptional regulation in lung macrophages during sub-acute Mycobacterium tuberculosis infection.
    Article Snippet: The quality of RNA was first checked for integrity on an Agilent TapeStation; samples with RNA integrity number (RIN) >7.0 were used for subsequent processing. .. Total RNA was subjected to two rounds of poly(A) selection using oligod(T)25 magnetic beads (New England Biolabs). .. An Illumina-compatible RNA-seq library was prepared using NEBNext Ultra RNA-seq Library Preparation Kit.

    Article Title: Directed evolution of glycopeptides using mRNA display
    Article Snippet: .. The protocol shown here is for 100 μL crude RNA-peptide fusion solution (~77 μL translation reaction followed by the addition of ~23 μL of potassium and magnesium containing solution), but can be scaled as desired. list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 To equilibrate beads, add 400 μL oligo(dT) binding buffer (high EDTA) to 100 μL Oligod(T) 25 Magnetic Beads (NEB) and vortex to wash. Place on a magnetic tube rack for 1 minute and discard supernatant. ..

    Article Title: FLL2 modulates Arabidopsis development and stress tolerance via polyadenylation and CPSF73 interaction
    Article Snippet: PhireHotstartII DNA Polymerase , Thermo , Cat# F122s. .. Oligod(T)25 Magnetic Beads , NEB , Cat# S1419S. .. SMARTScribe TM Reverse Transcriptase , Takara , Cat# 639538.

    Article Title: Modulation of alternative splicing by expression of small nuclear ribonucleoprotein polypeptide N.
    Article Snippet: .. The oligomers were first denatured at 95 °C for 5 min, rapidly chilled on ice, and incubated with Oligod(T)25 Magnetic Beads (New England Biolabs, Ipswich, MA, USA) in buffer D containing 2 mM MgCl2 and 0.1% NP-40 at room temperature for 30 min. After extensive washing to remove unbound oligomers, the nuclear extracts were then incubated with oligomer-bound beads at room temperature for 1 h, and then washed extensively. ..

    Article Title: The impact of thigmotaxis deprivation on the development of the German cockroach ( Blattella germanica )
    Article Snippet: RNAeasy® Mini Kit , Qiagen , Cat. No. 74106. .. oligod(T)25 magnetic beads , New England BioLabs , S1419S. .. ProtoScript® II First Strand cDNA Synthesis Kit , New England BioLabs , E6560S.

    Article Title: Directed evolution of glycopeptides using mRNA display
    Article Snippet: .. To equilibrate beads, add 400 μL oligo(dT) binding buffer (high EDTA) to 100 μL Oligod(T) 25 Magnetic Beads (NEB) and vortex to wash. Place on a magnetic tube rack for 1 minute and discard supernatant. ..

    other:

    Article Title: Glycogen synthase kinase-3 (GSK-3) activity regulates mRNA methylation in mouse embryonic stem cells
    Article Snippet: Gels were imaged using a Gel Doc XRS+ (Bio-Rad) using the 560DF50 filter.

    Binding Assay:

    Article Title: Directed evolution of glycopeptides using mRNA display
    Article Snippet: .. The protocol shown here is for 100 μL crude RNA-peptide fusion solution (~77 μL translation reaction followed by the addition of ~23 μL of potassium and magnesium containing solution), but can be scaled as desired. list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 To equilibrate beads, add 400 μL oligo(dT) binding buffer (high EDTA) to 100 μL Oligod(T) 25 Magnetic Beads (NEB) and vortex to wash. Place on a magnetic tube rack for 1 minute and discard supernatant. ..

    Article Title: Directed evolution of glycopeptides using mRNA display
    Article Snippet: .. To equilibrate beads, add 400 μL oligo(dT) binding buffer (high EDTA) to 100 μL Oligod(T) 25 Magnetic Beads (NEB) and vortex to wash. Place on a magnetic tube rack for 1 minute and discard supernatant. ..

    Incubation:

    Article Title: Modulation of alternative splicing by expression of small nuclear ribonucleoprotein polypeptide N.
    Article Snippet: .. The oligomers were first denatured at 95 °C for 5 min, rapidly chilled on ice, and incubated with Oligod(T)25 Magnetic Beads (New England Biolabs, Ipswich, MA, USA) in buffer D containing 2 mM MgCl2 and 0.1% NP-40 at room temperature for 30 min. After extensive washing to remove unbound oligomers, the nuclear extracts were then incubated with oligomer-bound beads at room temperature for 1 h, and then washed extensively. ..



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